- كلية الرافدين الجامعة مدير الموقع عمار محمود ابو الشعير

PATHOLOGY OF CELLULAR INJURY, DEGENERATION & NECROSISS بروفسيورعلي التميمي

PATHOLOGY LECTURE NOTES CELLULAR INJURY, DEGENERATION & NECROSISبروفسيورعلي التميمي ,استاذ الباثوفسيولوجي والتشريح المرضي

PATHOLOGY OF CELLULAR INJURY, DEGENERATION & NECROSIS بروفسيورعلي التميمي

Prof Ali Altimimi Professor of PathoPhysiology & Anatomical Pathology

,MbCHB,MSC,PHD,MD.Royal College London

محاضرات للاقسام الطبية لطلبة كلية الرافدين الجامعة

Learning objectives

At the end of this lecture, the student should be able to:

  1. Define hyperplasia, hypertrophy, atrophy, & Metaplasia & list some of their causes.
  2. Know the differences between reversible & irreversible forms of cell injury.
  3. Describe the mechanisms of necrosis.
  4. Describe the various types of necrosis & know some of their causes.

Cell injury underlies all diseases. So to understand diseases one, has to start by knowing what cell injury is. When a cell is exposed to an injurious agent (i.e. the causes of diseases discussed in chapter one), the possible outcomes are:

  1. The cell may adapt to the situation or
  2. They cell may acquire a reversible injury or
  3. The cell may obtain an irreversible injury & may die. The cell may die via one of two ways: either by necrosis or by apoptosis.

Which of these outcomes occur depends on both the injurious agent & on cellular factors. In other words, the result depends on the type, severity, & duration of the injury & on the type of the cell.

This chapter covers the types of cellular adaptation, reversible cell injury, & cell death in that order.

III. Types of cellular adaptation

The types of cellular adaptation include hypertrophy, atrophy, hyperplasia, & metaplasia.

  1. Hypertrophy

Hypertrophy is increase in the size of cells. Increased workload leads to increased protein synthesis & increased size & number of intracellular organelles which, in turn, leads to increased cell size. The increased cell size leads to increased size of the organ.

Examples: the enlargement of the left ventricle in hypertensive heart disease & the increase in skeletal muscle during sternous exercise.

  1. Hyperplasia

Hyperplasia is an increase in the number of cells. It can lead to an increase in the size of the organ. It is usually caused by hormonal stimulation. It can be physiological as in enlargement of the breast during pregnancy or it can pathological as in endometrial hyperplasia.

  1. Atrophy

Atrophy is a decrease in the size of a cell. This can lead to decreased size of the organ. The atrophic cell shows autophagic vacuoles which contain cellular debris from degraded organelles.

Atrophy can be caused by:

  1. Disuse

2.Undernutrition

  1. Decreased endocrine stimulation

4.Denervation

5.Old age

  1. Metaplasia

Metaplasia is the replacement of one differentiated tissue by another differentiated tissue. There are different types of metaplasia. Examples include:

1.Squamous metaplasia

This is replacement of another type of epithelium by squamous epithelium. For example, the columnar epithelium of the bronchus can be replaced by squamous epithelium in cigarette smokers

2.Osseous metaplasia

This replacement of a connective tissue by bone, for example at sites of injury.

  1. Reversible cellular changes & accumulations

Even though there are many different kinds of reversible cellular changes & accumulations, here we will only mention fatty change & accumulation of pigments.

  1. Fatty change

This is accumulation of triglycerides inside parenchymal cells. It is caused by an imbalance between the uptake, utilization, & secretion of fat. Fatty change is usually seen in the liver, heart, or kidney. Fatty liver may be caused by alcohol, diabetes mellitus, malnutrition, obesity, & poisonings. These etiologies cause accumulation of fat in the hepatocytes by the following mechanisms:

  1. Increased uptake of triglycerides into the parenchymal cells.
  2. Decreased use of fat by cells.
  3. Overproduction of fat in cells.
  4. Decreased secretion of fat from the cells.

2. The accumulations of pigments

  1. Cell death

Cells can die via one of the following two ways:

  1. Necrosis
  2. Apoptosis
  3. Necrosis

In necrosis, excess fluid enters the cell, swells it, & ruptures its membrane which kills it. After the cell has died, intracellular degradative reactions occur within a living organism. Necrosis does not occur in dead organisms. In dead organisms, autolysis & heterolysis take place.

Necrosis occurs by the following mechanisms:

  1. Hypoxia
  2. Free radical-induced cell injury
  3. Cell membrane damage
  4. Increased intracellular calcium level
  5. Hypoxia

Hypoxia is decreased oxygen supply to tissues. It can be caused by:

  1. Ischemia Ischemia is decreased blood flow to or from an organ. Ischemia can be caused by obstruction of arterial blood flow – the most common cause, or by decreased perfusion of tissues by oxygen-carrying blood as occurs in cardiac failure, hypotension, & shock.

2.Anemia Anemia is a reduction in the number of oxygen-carrying red blood cells.

3.Carbon monoxide poisoning

CO decreases the oxygen-capacity of red blood cells by chemical alteration of hemoglobin.

4.Poor oxygenation of blood due to pulmonary disease. The cell injury that results following hypoxia can be divided into early & late stages:

  1. Early (reversible) stages of hypoxic cell injury

At this stage, hypoxia results in decreased oxidative phosphorylation & ATP synthesis. Decreased ATP leads to:

  1. Failure of the cell membrane Na – K pump, which leads to increased intracellular Na & water, which cause cellular & organelle swelling. Cellular swelling (hydropic change) is characterized by the presence of large vacuoles in the cytoplasm. The endoplasmic reticulum also swells. The mitochondria show a low amplitude swelling. All of the above changes are reversible if the hypoxia is corrected.

b.Disaggregation of ribosomes & failure of protein synthesis.

  1. Late (irreversible) stages of hypoxic cell injury.

This is caused by severe or prolonged injury. It is caused by  massive calcium influx & very low pH, which lead to activation of enzymes, which damage the cell membrane& organelle membranes. Irreversible damage to the mitochondria, cell membranes, & the nucleus mark the point of no return for the cell, that is after this stage, the cell is destined to die.

Release of aspartate aminotransferase (AST), creatine phosphokinase(CPK), & lactate dehydrogenase (LDH) into the blood is an important indicator of irreversible injury to heart muscle following myocardial infarction.

  1. Free radical-induced injury

Free radical is any molecule with a single unpaired electron in the outer orbital. Examples include superoxide & the hydroxyl radicals. Free radicals are formed by normal metabolism, oxygen toxicity, ionizing radiation, & drugs & chemicals, & reperfusion injury. They are degraded by spontaneous decay, intracellular enzymes such as glutathione peroxidase, catalase, or superoxide dismutase, & endogenous substances such as ceruloplasmin or transferrin. When the production of free radicals exceeds their degradation, the excess free radicals cause membrane pump damage, ATP depletion, & DNA damage. These can cause cell injury & cell death.

  1. Cell membrane damage

Direct cell membrane damage as in extremes of temprature, toxins, or viruses, or indirect cell membrane damage as in the case of hypoxia can lead to cell death by disrupting the homeostasis of the cell.

  1. Increased intracellular calcium level

    Types of necrosis

    The types of necrosis include:

    1. Coagulative necrosis

    2.Liquefactive necrosis

    3.Fat necrosis

    4.Caseous necrosis

    5.Gangrenous necrosis

    1. Coagulative necrosis

    Cogulative necrosis most often results from sudden interruption of blood supply to an organ, especially to the heart. It is, in early stages, characterized by general preservation of tissue architecture. It is marked by the following nuclear changes: Pyknosis (which is chromatin clumping & shrinking with increased basophilia), karyorrhexis (fragmentation of chromatin), & karyolysis (fading of the chromatin material).

    1. Liquefactive necrosis

    Liquefactive necrosis is characterized by digestion of tissue. It shows softening & liquefaction of tissue. It characteristically results from ischemic injury to the CNS. It also occurs in suppurative infections characterized by formation of pus.

    1. Fat necrosis

    Fat necrosis can be caused by trauma to tissue with high fat content, such as the breast or it can also be caused by acute hemorrhagic pancreatitis in which pancreatic enzymes diffuse into the inflamed pancreatic tissue & digest it. The fatty acids released from the digestion form calcium salts (soap formation or dystrophic calcification). In addition, the elastase enzyme digests the blood vessels & cause the hemorrhage inside the pancreas, hence the name hemorrhagic pancreatitis.

    1. Caseous necrosis

    Caseous necrosis has a cheese-like (caseous, white) appearance to the naked eye. And it appears as an amorphous eosinophilic material on microscopic examination. Caseous necrosis is typical of tuberculosis.

    1. Gangrenous necrosis

    This is due to vascular occlusion & most often affects the lower extremities & the bowel. It is called wet gangrene if it is complicated by bacterial infection which leads to superimposed liquefactive necrosis. Whereas it is called dry gangrene if there is only coagulative necrosis without liquefactive necrosis.

    1. Apoptosis

    Apoptosis is the death of single cells within clusters of other cells. (Note that necrosis causes the death of clusters of cells.) In apoptosis, the cell shows shrinkage & increased acidophilic staining of the cell. This is followed by fragmentation of the cells. These fragments are called apoptotic bodies. Apoptosis usually occurs as a physiologic process for removal of cells during embryogenesis, menstruation, etc… It can also be seen in pathological conditions caused by mild injurious agents.

    Apoptosis is not followed by inflammation or calcification. The above mentioned features distinguish apoptosis from necrosis.

    1. Pathologic calcification

    Pathologic calcification is divided into 2 types:

    1. Metastatic calcification

    This is caused by hypercalcemia, resulting from hyperparathyroidism, milk-alkali syndrome, sarcoidosis etc…

    1. Dystrophic calcification

    This occurs in previously damaged tissue, such as areas of old trauma, tuberculous lesions, scarred heart valves, & atherosclerotic lesions.

    Unlike metastatic calcification, it is not caused by hypercalcemia. Typically, the serum calcium level is normal.

    Exercises

    1. Why should you study cell injury?
    2. Compare & contrast the various types of cellular adaptation.
    3. Describe the various mechanisms of necrosis.
    4. Mention some of the causes of each of the various types of necrosis.
    5. Compare & contrast necrosis & apoptosis.
    6. Contrast metastatic & dystrophic calcification.